ABSTRACT
Background: Advances of nanotechnology have led to the development of nanomaterials with both potential diagnostic and therapeutic applications. Among them, Super Paramagnetic Iron Oxide Nanoparticles [SPIONs] have received particular attention. Modified EDC coupling fraction was used to fabricate the SPION-C595 as an MR imaging contrast agent for breast cancer detection in early stages
Methods: Nanoprobe characterization was confirmed using Fourier Transform Infrared Spectroscopy [FT-IR], Scanning Electron Microscopy with Energy Dispersive X-Ray Spectroscopy [SEM-EDAX], and Photon Correlation Spectroscopy [PCS]. Protein and iron concentration of nanoprobe was examined by standard method. MTT assay was performed to evaluate the cytotoxicity of the nanoprobe in breast cancer cell line [MCF-7]. T[2]-weighted MR imaging was performed to evaluate the signal enhancement on T[2] relaxation time of nanoprobe using spin-echo pulse sequence
Results: As results showed, SPIONs-C595 provided active targeting of breast cancer cell [MCF-7] at a final concentration of 600 micro gFe/ml. The final concentration of protein was calculated to be at 0.78 micro gprotein/ml. The hydrodynamic size of the nanoprobe was 87.4+/-0.7 nm. The MR imaging results showed a good reduction of T[2] relaxation rates for the highest dose of SPIONs-C595
Discussion: Based on the results, SPIONs-C595 nanoprobe has a potential in T[2]- weighted MR imaging contrast agent for breast cancer cell [MCF-7] detection
ABSTRACT
Background: Magnetic resonance imaging [MRI] plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody conjugated super paramagnetic iron oxide nanoparticles [SPIONs-C595] for the detection of breast cancer cell [MCF-7]
Methods: The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy. The selectivity of the nanoprobe for breast cancer cells [MCF-7] was obtained by Prussian blue, atomic emission spectroscopy, and MRI relaxometry
Results: The in vitro MRI showed that T2 relaxation time will be reduced 76% when using T2-weighed magnetic resonance images compared to the control group [untreated cells] at the dose of 200 micro g Fe/ml, as the optimum dose. In addition, the results showed the high uptake of nanoprobe into MCF-7 cancer cells
Conclusion: The SPIONs-C595 nanoprobe has potential for the detection of specific breast cancer